BCL2L1 antibody (AA 3-14)

Catalog No. ABIN3025808

This Mouse Monoclonal antibody specifically detects BCL2L1 in WB, IF, FACS and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Pig.

Quick Overview for BCL2L1 antibody (AA 3-14) (ABIN3025808)

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Reactivity: Human, Mouse, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL2L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: BX006-2H12

Product Details anti-BCL2L1 Antibody

Binding Specificity: AA 3-14

Purification: Protein G affinity chromatography

Immunogen: Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this Bcl-X antibody.

Isotype: IgG2a

Application Details

Application Notes: The optimal dilution of the Bcl-X antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Bcl-X antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for BCL2L1

Target: BCL2L1 (BCL2-Like 1 (BCL2L1))

Alternative Name: Bcl-X

Background: Recognizes a protein of 27  kDa, identified as the Bcl-X protein. This mAb cocktail shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling