NKX2-1 antibody
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- Target See all NKX2-1 Antibodies
- NKX2-1 (NK2 Homeobox 1 (NKX2-1))
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Reactivity
- Human, Mouse, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NKX2-1 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- Full length rat recombinant protein was used as the immunogen for this NKX2.1 antibody.
- Clone
- SPM150
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product NKX2-1 Primary Antibody
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- Application Notes
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The optimal dilution of the NKX2.1 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the NKX2.1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- NKX2-1 (NK2 Homeobox 1 (NKX2-1))
- Alternative Name
- NKX2.1 (NKX2-1 Products)
- Synonyms
- BCH antibody, BHC antibody, NK-2 antibody, NKX2.1 antibody, NKX2A antibody, TEBP antibody, TITF1 antibody, TTF-1 antibody, TTF1 antibody, titf1-A antibody, Xnkx-2.1 antibody, NKX2-1 antibody, LOC100227803 antibody, AV026640 antibody, Nkx2.1 antibody, T/EBP antibody, Titf1 antibody, Ttf-1 antibody, nk2.1a antibody, nkx2.1 antibody, nkx2.4a antibody, titf1 antibody, titf1a antibody, NK2 homeobox 1 antibody, NK2 homeobox 1 L homeolog antibody, NK2 homeobox 4b antibody, NKX2-1 antibody, nkx2-1.L antibody, Nkx2-1 antibody, nkx2.4b antibody
- Background
- Recognizes a protein of 40 kDa, identified as NKX2.1 or Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-NKX2.1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of NKX2.1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. Reactivity is also seen in thyroid malignancies.
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour
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