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CD16 antibody

This anti-CD16 antibody is a Mouse Monoclonal antibody detecting CD16 in FACS, IF and Func. Suitable for Human.

SDS-PAGE Analysis of Purified, BSA-Free CD16 Antibody (clone HuNK2). (CD16 antibody)

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Catalog No. ABIN3025888

Quick Overview for CD16 antibody (ABIN3025888)

Target

See all CD16 Antibodies

CD16

Reactivity

144
31
20
20
20
9
8
8
8
5
3
3
2
2
2
2
2
2
2
2
2
2

Human

Host

Mouse

Clonality

Monoclonal

Conjugate

This CD16 antibody is un-conjugated

Application

144
36
28
28
27
24
23
18
6
6
5
4
4
2
2
2
1
1
1
1

Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Clone

HuNK2

Discover our top product CD16 Primary Antibody

Purification

Protein G affinity chromatography

Immunogen

Human peripheral blood lymphocytes from a NK-leukemia patient were used as the immunogen for the CD16 antibody.

Isotype

IgG2a kappa
anti-CD16 (CD16) antibody
CD16 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal 3G8 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human, Non-Human Primate FACS, IP Host: Mouse Monoclonal LNK16 unconjugated

anti-CD16 (CD16) antibody (FITC)
CD16 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal LNK16 FITC

anti-CD16 (CD16) antibody
CD16 Reactivity: Human FACS, IP, WB, Func Host: Mouse Monoclonal MEM-154 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human, Pig, Non-Human Primate FACS Host: Mouse Monoclonal MEM-168 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human FACS, IP, WB Host: Mouse Monoclonal MEM-154 unconjugated

anti-CD16 (CD16) antibody (Biotin)
CD16 Reactivity: Human FACS, IP, WB Host: Mouse Monoclonal MEM-154 Biotin

anti-CD16 (CD16) (AA 17-208) antibody
CD16 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 2G10A9 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human, Non-Human Primate FACS, IP, Func, IHC (fro) Host: Mouse Monoclonal 3G8 unconjugated

anti-CD16 (CD16) antibody
CD16 Reactivity: Human FACS Host: Mouse Monoclonal GRM1 unconjugated

Application Notes

Optimal dilution of the CD16 antibody should be determined by the researcher.\. Functional Activity (Order SAF formulation),Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative

Azide free

Storage

4 °C,-20 °C

Storage Comment

Store the CD16 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Target

CD16

Alternative Name

CD16

Background

Recognizes a protein of 50-65  kDa, identified as CD16 (Workshop IV, Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This mAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils.

Pathways

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process

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