CD55 antibody

Catalog No. ABIN3026018

This Mouse Monoclonal antibody specifically detects CD55 in FACS, IF and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for CD55 antibody (ABIN3026018)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 143-30

Product Details anti-CD55 Antibody

Purification: Protein G affinity chromatography

Immunogen: PHA stimulated human PBL were used as the immunogen for the CD55 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the CD55 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD55 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55 (DAF)

Background: Recognizes a single chain glycoprotein of 70  kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The mAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity