Granulocytes antibody

Catalog No. ABIN3026077

The Mouse Monoclonal anti-Granulocytes antibody (Clone SPM250) (ABIN3026077) specifically detects Granulocytes in FACS, IHC (p) and IF. The antibody is reactive with Human and Macaque samples.

Quick Overview for Granulocytes antibody (ABIN3026077)

Target: Granulocytes

Reactivity: Human, Macaque

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: SPM250

Product Details

Purification: Protein G affinity chromatography

Immunogen: Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes were used as the immunogen for this Granulocyte antibody.

Isotype: IgG1

Application Details

Application Notes: The optimal dilution of the Granulocyte antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Granulocyte antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Granulocytes

Alternative Name: Granulocyte

Background: Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. SPM250 Ab reacts with early precursor and mature forms of human myeloid cells. This mAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.