Macrophages antibody

Catalog No. ABIN3026091

The Mouse Monoclonal anti- antibody has been validated for FACS, IHC (p) and IF. It is suitable to detect in samples from Human, Rat, Mouse, Pig, Monkey, Cat, Cow, Dog, Baboon, Goat, Guinea Pig, Horse and Rabbit.

Quick Overview for Macrophages antibody (ABIN3026091)

Target: Macrophages

Reactivity: Human, Rat, Mouse, Pig, Monkey, Cat, Cow, Dog, Baboon, Goat, Guinea Pig, Horse, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: SPM281

Product Details

Purification: Protein G affinity chromatography

Immunogen: An affinity purified monocyte membrane preparation was used as the immunogen for the anti-Macrophage antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the anti-Macrophage antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Macrophage antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Macrophages

Alternative Name: Macrophage

Background: Recognizes the macrophage L1 protein Calprotectin, expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.