RBP1 antibody (AA 74-182)

Catalog No. ABIN3026095

This anti-RBP1 antibody is a Mouse Monoclonal antibody detecting RBP1 in IF and IHC (p). Suitable for Human, Mouse, Rat, Rabbit, Monkey and Goat.

Quick Overview for RBP1 antibody (AA 74-182) (ABIN3026095)

Target: RBP1 (Retinol Binding Protein 1, Cellular (RBP1))

Reactivity: Human, Mouse, Rat, Rabbit, Monkey, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBP1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: G4E4

Product Details anti-RBP1 Antibody

Binding Specificity: AA 74-182

Purification: Protein G affinity chromatography

Immunogen: Retinol binding protein purified from human plasma was used as the immunogen for this RBP antibody. Its epitope localizes between amino acids 74-182.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. IHC (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2),IF: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the RBP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for RBP1

Target: RBP1 (Retinol Binding Protein 1, Cellular (RBP1))

Alternative Name: RBP1 / Retinol Binding Protein 1

Background: Retinol binding protein a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. RBP1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.

Gene ID: 5947