FOXP3 antibody (N-Term)

Catalog No. ABIN3026198

This Mouse Monoclonal antibody specifically detects FOXP3 in FACS, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for FOXP3 antibody (N-Term) (ABIN3026198)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: FHBP3-1

Product Details anti-FOXP3 Antibody

Binding Specificity: N-Term

Purification: Protein G affinity chromatography

Immunogen: An N-terminal recombinant protein fragment was used as the immunogen for this FOXP3 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Titering of the FOXP3 antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris buffer with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the FOXP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FOXP3

Background: The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 is essential for normal immune homeostasis. It represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

Gene ID: 50943

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation