Blood Group Lewis B antibody

Catalog No. ABIN3026216

This Mouse Monoclonal antibody specifically detects Blood Group Lewis B in IF and IHC (p). It exhibits reactivity toward Human and Guinea Pig.

Quick Overview for Blood Group Lewis B antibody (ABIN3026216)

Target: Blood Group Lewis B

Reactivity: Human, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Blood Group Lewis B antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2-25LE or LWB01

Product Details anti-Blood Group Lewis B Antibody

Purification: Protein G affinity chromatography

Immunogen: Mucin isolated from human ovarian cyst fluid was used as the immunogen for this Lewis b antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The optimal dilution of the Blood Group Lewis b antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Lewis b antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Blood Group Lewis B

Target: Blood Group Lewis B

Alternative Name: Lewis B

Background: The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers.