Blood Group Lewis B antibody
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- Target See all Blood Group Lewis B products
- Blood Group Lewis B
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Reactivity
- Human, Guinea Pig
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Blood Group Lewis B antibody is un-conjugated
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Application
- Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- Mucin isolated from human ovarian cyst fluid was used as the immunogen for this Lewis b antibody.
- Clone
- 2-25LE or LWB01
- Isotype
- IgG1 kappa
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anti-Blood Group Lewis B antibody
Reactivity: Human IHC (p), WB, EIA Host: Mouse Monoclonal 2-25LE unconjugated
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- Application Notes
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The optimal dilution of the Blood Group Lewis b antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- Preservative
- Azide free
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the Lewis b antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- Blood Group Lewis B
- Alternative Name
- Lewis B (Blood Group Lewis B Products)
- Background
- The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers.
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