PAX7 antibody (AA 300-600)

Catalog No. ABIN3026252

This Mouse Monoclonal antibody specifically detects PAX7 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for PAX7 antibody (AA 300-600) (ABIN3026252)

Target: PAX7 (Paired Box 7 (PAX7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX7 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PBPX7

Product Details anti-PAX7 Antibody

Binding Specificity: AA 300-600

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment (aa300-600) was used as the immunogen for the PAX7 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the PAX7 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris buffer with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the PAX7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: PAX7

Background: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).