TGFA antibody (AA 34-43)

Catalog No. ABIN3026449

This anti-TGFA antibody is a Mouse Monoclonal antibody detecting TGFA in IF, FACS and IHC (p). Suitable for Human, Rabbit and Zebrafish (Danio rerio).

Quick Overview for TGFA antibody (AA 34-43) (ABIN3026449)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human, Rabbit, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: P-T1

Product Details anti-TGFA Antibody

Binding Specificity: AA 34-43

Characteristics: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Purification: Protein G affinity chromatography

Immunogen: Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGF-alpha antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the TGF-alpha antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the TGF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF-alpha

Background: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway