CD68 antibody

Catalog No. ABIN3026529

The Mouse Monoclonal anti-CD68 antibody has been validated for FACS, IF and IHC (p). It is suitable to detect CD68 in samples from Human, Monkey, Cat and Rabbit.

Quick Overview for CD68 antibody (ABIN3026529)

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Human, Monkey, Cat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD68 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM130

Product Details anti-CD68 Antibody

No Cross-Reactivity: Pig (Porcine), Dog (Canine), Chicken

Characteristics: This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Purification: Protein G affinity chromatography

Immunogen: A human alveolar macrophage subcellular fraction was used as the immunogen for this anti-CD68 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The optimal dilution of the anti-CD68 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-CD68 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68

Background: This antibody recognizes a glycoprotein of 110  kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.