CD34 antibody

Catalog No. ABIN3026669

This Mouse Monoclonal antibody specifically detects CD34 in FACS and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for CD34 antibody (ABIN3026669)

Target: CD34

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ICO-115

Product Details anti-CD34 Antibody

Characteristics: This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Purification: Protein G affinity chromatography

Immunogen: Blast cells from a chronic myeloid leukemia patient were used as the immunogen for this CD34 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD34 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120  kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

Gene ID: 947