alpha Fetoprotein antibody

Catalog No. ABIN3026704

This anti-alpha Fetoprotein antibody is a Mouse Monoclonal antibody detecting alpha Fetoprotein in IF, FACS and IHC (p). Suitable for Human, Mouse, Rat, Pig, Dog, Monkey and Cat.

Quick Overview for alpha Fetoprotein antibody (ABIN3026704)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human, Mouse, Rat, Pig, Dog, Monkey, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: C3

Product Details anti-alpha Fetoprotein Antibody

No Cross-Reactivity: Cow (Bovine), Cat (Feline), Mouse (Murine), Rat (Rattus)

Characteristics: This antibody recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as Alpha fetoprotein (AFP). The AFP antibody is highly specific and shows no cross-reaction with other oncofetal antigens or serum albumin. Alpha fetoprotein is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. AFP antibody has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Purification: Protein G affinity chromatography

Immunogen: Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the AFP antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the AFP antibody at 2-8°C.

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP alpha Fetoprotein

Background: This antibody recognizes an oncofetal glycoprotein with a single chain of 70  kDa, which is identified as Alpha fetoprotein (AFP). The AFP antibody is highly specific and shows no cross-reaction with other oncofetal antigens or serum albumin. Alpha fetoprotein is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. AFP antibody has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Gene ID: 174

Pathways: C21-Steroid Hormone Metabolic Process