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KRT1 antibody

KRT1 Reactivity: Human, Rat, Mouse WB, IF, FACS, IHC (p) Host: Mouse Monoclonal 34BE12 unconjugated
Catalog No. ABIN3026726
  • Target See all KRT1 Antibodies
    KRT1 (Cytokeratin HMW (KRT1))
    Reactivity
    • 27
    • 27
    • 18
    • 10
    • 10
    • 10
    • 10
    • 9
    • 1
    Human, Rat, Mouse
    Host
    • 27
    Mouse
    Clonality
    • 27
    Monoclonal
    Conjugate
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    This KRT1 antibody is un-conjugated
    Application
    • 26
    • 26
    • 17
    • 16
    • 9
    • 9
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Characteristics
    This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.
    Purification
    Protein G affinity chromatography
    Immunogen
    Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.
    Clone
    34BE12
    Isotype
    IgG1 kappa
  • Application Notes
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HMW Cytokeratin antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells,IF: 0.5-1.0 μg/mL,WB: 0.5-1.0 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the HMW Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    KRT1 (Cytokeratin HMW (KRT1))
    Alternative Name
    HMW Cytokeratin (KRT1 Products)
    Synonyms
    CK1 antibody, EHK antibody, EHK1 antibody, EPPK antibody, K1 antibody, KRT1A antibody, NEPPK antibody, keratin 1 antibody, KRT1 antibody
    Background
    This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.
    Gene ID
    3848
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