Nucleoli Marker antibody

Catalog No. ABIN3026802

The Mouse Monoclonal anti-Nucleoli Marker antibody is suitable to detect Nucleoli Marker in samples from Human. It has been validated for IF, FACS, ICC and IHC (p).

Quick Overview for Nucleoli Marker antibody (ABIN3026802)

Target: Nucleoli Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NM95

Product Details

Characteristics: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Purification: Protein G affinity chromatography

Immunogen: Nuclei of myeloid leukemia biopsy cells were used as the immunogen.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nucleoli marker antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 0.5-1 μg/mL,Immunocytochemistry (Acetone-fixed): 0.5-1 μg/mL for 30 min at RT,IHC (Formalin-paraffin): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Nucleoli marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Nucleoli Marker

Background: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.