USP18 antibody

Catalog No. ABIN302692

This Rabbit Polyclonal antibody specifically detects USP18 in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for USP18 antibody (ABIN302692)

Target: USP18 (Ubiquitin Specific Peptidase 18 (USP18))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP18 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-USP18 Antibody

Specificity: This antibody reacts to amino acids 40 to 49 of human USP18.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide

Application Details

Application Notes: Immunofluorescence. Western Blot: 1: 500 - 1: 2000. Immunohistochemistry: 5 μg/mL. ELISA: 1: 10000 - 1: 50000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.93 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for USP18

Target: USP18 (Ubiquitin Specific Peptidase 18 (USP18))

Alternative Name: USP18

Background: UBP43 (also known as ISG43, Ubl thiolesterase 18, ISG15-specific processing protease, 43 kDa ISG15-specific protease, ubiquitin specific protease 18 or USP18) is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.Synonyms: 43 kDa ISG15-specific protease, ISG15-specific-processing protease, ISG43, Ubl carboxyl-terminal hydrolase 18, Ubl thioesterase 18

Gene ID: 11274

NCBI Accession: NP_059110

UniProt: Q9UMW8