Secretory Component Glycoprotein antibody

Catalog No. ABIN3026937

The Mouse Monoclonal anti-Secretory Component Glycoprotein antibody has been validated for IHC (p), IF and FACS. It is suitable to detect Secretory Component Glycoprotein in samples from Human and Rat.

Quick Overview for Secretory Component Glycoprotein antibody (ABIN3026937)

Target: Secretory Component Glycoprotein

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Secretory Component Glycoprotein antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: SPM217

Product Details anti-Secretory Component Glycoprotein Antibody

Characteristics: This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Purification: Protein G affinity chromatography

Immunogen: Secretory Component protein isolated from human colostrum was used as the immunogen for this Secretory Component antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The optimal dilution of the Secretory Component antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Secretory Component antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Secretory Component Glycoprotein

Target: Secretory Component Glycoprotein

Background: This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.