Ubiquitin antibody (AA 29-45)

Catalog No. ABIN3029417

The Rabbit Polyclonal anti-Ubiquitin antibody has been validated for WB and IHC (p). It is suitable to detect Ubiquitin in samples from Human, Mouse and Rat.

Quick Overview for Ubiquitin antibody (AA 29-45) (ABIN3029417)

Target: Ubiquitin (Ub)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Ubiquitin Antibody

Binding Specificity: AA 29-45

Purification: Antigen affinity

Immunogen: Amino acids 29-45 (KIQDKEGIPPDQQRLIF) were used as the immunogen for this Ubiquitin antibody (100% human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Ubiquitin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background: Ubiquitin(originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues(ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway