EPH Receptor B3 antibody (C-Term)

Catalog No. ABIN3029711

The Rabbit Polyclonal anti-EPH Receptor B3 antibody is suitable to detect EPH Receptor B3 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for EPH Receptor B3 antibody (C-Term) (ABIN3029711)

Target: EPH Receptor B3 (EPHB3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EPH Receptor B3 Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Eph receptor B3 (SIQDMRLQMNQTLPVQV) was used as the immunogen for this EphB3 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the EphB3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the EphB3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for EPH Receptor B3

Target: EPH Receptor B3 (EPHB3)

Alternative Name: EphB3 Ephrin Receptor B3

Background: Ephrin Receptor B3, is also known as human embryo kinase2 (HEK2) or Eph-like tyrosine kinase 2 (ETK2) is a member of the EPH/ELK family of tyrosine kinases. The gene encodes a 998-amino acid polypeptide having a single putative transmembrane domain, a secretory signal sequence, and two fibronectin repeats. The gene is mapped to human chromosome 3q21-qter. EphB3 interacts with two ligands of EPH-related kinases (LERKs), LERK2/EFNB1 and LERK5/EFNB2. Coincubation of EphB3- and LERK2-expressing cells induces cell-cell adhesion and aggregation. Additionally, coexpression of the two proteins results in reduced kinase activity of EphB3.

UniProt: P54753

Pathways: RTK Signaling