Proprotein Convertase 1/3 (C-Term) antibody

Catalog No. ABIN3029753

The Rabbit Polyclonal anti-Proprotein Convertase 1/3 antibody is suitable to detect Proprotein Convertase 1/3 in samples from Human, Mouse and Rat. It has been validated for IHC (p) and WB.

Quick Overview for Proprotein Convertase 1/3 (C-Term) antibody (ABIN3029753)

Target: Proprotein Convertase 1/3

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Proprotein Convertase 1/3 (DVFYNTKPYKHRDDR) was used as the immunogen for this Proprotein Convertase 1/3 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Proprotein Convertase 1/3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Proprotein Convertase 1/3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details

Target: Proprotein Convertase 1/3

Background: Proprotein Convertase, Subtilisin/Kexin-Type, 1, also known as PC1 or NEC1, is an enzyme that in humans is encoded by the PCSK1 gene. It is a neuroendocrine convertase that belongs to a family of subtilisin-like serine endoproteases that process large precursor proteins into mature bioactive products. By in situ hybridization, Seidah et al.(1991) mapped the gene to human chromosome 5q15-q21 and mouse chromosome 13. Ohagi et al.(1996) noted that PCSK1 initiates the sequential processing of proinsulin to insulin by cleaving the proinsulin molecule on the C-terminal side of the dibasic peptide, arg31-arg32, joining the B-chain and C-peptide. By observing the phenotypic features in patients with PCSK1 mutations, Jackson et al.(2003) concluded that human intestinal absorptive function is dependent on the enzymes activity.

UniProt: P29120