Annexin a1 antibody (AA 2-346)

Catalog No. ABIN3029968

This Rabbit Polyclonal antibody specifically detects Annexin a1 in WB, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Annexin a1 antibody (AA 2-346) (ABIN3029968)

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin a1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Annexin a1 Antibody

Binding Specificity: AA 2-346

Purification: Antigen affinity

Immunogen: Human partial recombinant protein (AA 2-346) was used as the immunogen for this Annexin A1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Annexin A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Annexin A1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Alternative Name: Annexin A1

Background: ANXA1, also known as Lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. It belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

Gene ID: 301

Pathways: Hormone Transport