BUB1 antibody (AA 731-1085)

Catalog No. ABIN3030212

The Rabbit Polyclonal anti-BUB1 antibody is suitable to detect BUB1 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for BUB1 antibody (AA 731-1085) (ABIN3030212)

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BUB1 Antibody

Binding Specificity: AA 731-1085

Purification: Antigen affinity

Immunogen: Human partial recombinant protein (AA 731-1085) was used as the immunogen for this Bub1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Bub1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Bub1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: Bub1

Background: Budding uninhibited by benzimidazoles 1, also known as Mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It was first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of Bub1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. Bub1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.

Gene ID: 699