CAPN1 antibody (Middle Region)

Catalog No. ABIN3030267

The Rabbit Polyclonal anti-CAPN1 antibody (ABIN3030267) specifically detects CAPN1 in WB, ICC, IHC (p) and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CAPN1 antibody (Middle Region) (ABIN3030267)

Target: CAPN1 (Calpain 1 (CAPN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CAPN1 Antibody

Binding Specificity: Middle Region

Purification: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human CAPNL1 (EWNNVDPYERDQLRV) was used as the immunogen for this Calpain 1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Calpain 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Calpain 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Alternative Name: Calpain 1

Background: Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, CAPN1 (mu-calpain) and CAPN2 (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 82 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of Calpain1/2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CAPNL1 to chromosome 11.

UniProt: P07384