CASP14 antibody (AA 223-242)

Catalog No. ABIN3030293

The Rabbit Polyclonal anti-CASP14 antibody is suitable to detect CASP14 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (p).

Quick Overview for CASP14 antibody (AA 223-242) (ABIN3030293)

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASP14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CASP14 Antibody

Binding Specificity: AA 223-242

Purification: Antigen affinity

Immunogen: Amino acids 223-242 (KARKTNPEIQSTLRKRLYLQ-human) were used as the immunogen for this Caspase-14 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Caspase-14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Caspase-14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CASP14

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Alternative Name: Caspase-14

Background: Caspase 14 is an enzyme that in humans is encoded by the CASP14 gene. CASP14 belongs the evolutionarily conserved caspase family. CASP14 was assigned to chromosome 19p13.1. Using Northern blot and semiquantitative RT-PCR analyses, it was showed that CASP14 was transcriptionally upregulated during maintenance of confluent cultures of human epidermal keratinocytes. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. Caspase 14 seems to play a role in keratinocyte differentiation and cornification and probably regulates maturation of the epidermis by proteolytically processing filaggrin.

UniProt: P31944