Cullin 1 antibody (N-Term)

Catalog No. ABIN3030576

This anti-Cullin 1 antibody is a Rabbit Polyclonal antibody detecting Cullin 1 in WB and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Cullin 1 antibody (N-Term) (ABIN3030576)

Target: Cullin 1 (CUL1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cullin 1 Antibody

Binding Specificity: N-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human Cullin 1 (RQSMAKSRYMELYTHVYNY) was used as the immunogen for this CUL1 antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the CUL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CUL1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Cullin 1

Target: Cullin 1 (CUL1)

Alternative Name: CUL1

Background: Cullin 1 is a human protein and gene from cullin family. This protein plays an important role in protein degradation and protein ubiquitination. This is an essential component of the SCF(SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Human CUL1, but not the other closely related cullins 2, 3, 4A, and 5, selectively interacts with human SKP1.

UniProt: Q13616

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size