Cyclin B1 antibody (AA 1-433)

Catalog No. ABIN3030583

This Rabbit Polyclonal antibody specifically detects Cyclin B1 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Cyclin B1 antibody (AA 1-433) (ABIN3030583)

Target: Cyclin B1 (CCNB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Cyclin B1 Antibody

Binding Specificity: AA 1-433

Purification: Antigen affinity

Immunogen: Human partial recombinant protein (AA 1-433) was used as the immunogen for this Cyclin B1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Cyclin B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Cyclin B1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background: Cyclin B1 is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. It is a regulatory protein involved in mitosis. The gene product complexes with< a href=../search_result.php?search_txt=cdc2>p34(Cdc2)to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB1 contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the< a href=../search_result.php?search_txt=cyclin b1>Cyclin B1-< a href=../search_result.php?search_txt=cdk1>Cdk1complex is activated, it is not deactivated.

Gene ID: 891

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase