Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (C-Term) antibody

Details for Product No. ABIN3030680
Target
Binding Specificity
C-Term
16
13
10
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
93
64
39
6
4
4
4
4
3
2
2
2
2
2
2
1
1
1
Host
Rabbit
72
21
7
Clonality
Polyclonal
Conjugate
Un-conjugated
5
4
4
4
4
4
4
3
3
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
60
30
26
24
22
16
11
7
5
3
1
Options
Immunogen An amino acid sequence from the C-terminus of human Aromatase (KNMLEMIFTPRNSDRCLEH) was used as the immunogen for this Aromatase antibody.
Isotype IgG
Purification Antigen affinity
Target
Alternative Name Aromatase (CYP19A1 Antibody Abstract)
Background Aromatase is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.
UniProt P11511
Pathways Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis
Application Notes The stated application concentrations are suggested starting amounts. Titration of the Aromatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Restrictions For Research Use only
Buffer 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage -20 °C
Storage Comment After reconstitution, the Aromatase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Images
Image no. 1 for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (C-Term) antibody (ABIN3030680) IHC-P: Aromatase antibody testing of human placenta tissue
Image no. 2 for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (C-Term) antibody (ABIN3030680) Western blot testing of Aromatase antibody and Lane 1: human placenta; 2: human pla...
Image no. 3 for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (C-Term) antibody (ABIN3030680) Western blot testing of Aromatase antibody and Lane 1: human placenta