Aromatase antibody (Internal Region)

Catalog No. ABIN238672

This Goat Polyclonal antibody specifically detects Aromatase in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Aromatase antibody (Internal Region) (ABIN238672)

Target: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Aromatase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Aromatase Antibody

Binding Specificity: Internal Region

Purpose: Aromatase / CYP19A1

Sequence: HDLSLHPDET KN

Specificity: This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1), which are identical

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HDLSLHPDETKN, from the internal region of the protein sequence according to NP_000094.2, NP_112503.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 4 μg/mL.

Western Blot: Approx 50-55 kDa band observed in Human Placenta lysates (calculated MW of 57.9 kDa according to NP_000094.2 and NP_112503.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Aromatase

Target: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Alternative Name: CYP19A1

Background: CYP19A1, cytochrome P450, family 19, subfamily A, polypeptide 1 , ARO, ARO1, CPV1, CYAR, CYP19, MGC104309, P-450AROM, aromataseN: cytochrome P450, family 19, cytochrome P450, subfamily XIX (aromatization of androgens), estrogen synthetase, flavoprotein-li

Gene ID: 1588

NCBI Accession: NP_000094, NP_112503

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis