Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (Internal Region) antibody

Details for Product No. ABIN238672
Target
Binding Specificity
Internal Region
16
13
9
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
92
64
39
6
4
4
4
4
3
2
2
2
2
2
2
1
1
1
Host
Goat
72
21
6
Clonality
Polyclonal
Conjugate
Un-conjugated
5
4
4
4
4
4
4
3
3
2
2
2
2
2
2
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
59
29
26
23
22
16
11
7
5
3
1
Options
Purpose Aromatase / CYP19A1
Immunogen Peptide with sequence C-HDLSLHPDETKN, from the internal region of the protein sequence according to NP_000094.2, NP_112503.1.
Blocking Peptide Blocking peptide for this product available: ABIN369152
Sequence HDLSLHPDET KN
Isotype IgG
Specificity This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1), which are identical
Predicted Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name CYP19A1 (CYP19A1 Antibody Abstract)
Background CYP19A1, cytochrome P450, family 19, subfamily A, polypeptide 1 , ARO, ARO1, CPV1, CYAR, CYP19, MGC104309, P-450AROM, aromataseN: cytochrome P450, family 19, cytochrome P450, subfamily XIX (aromatization of androgens), estrogen synthetase, flavoprotein-linked monooxygenase, microsomal monooxygenase
Gene ID 1588
NCBI Accession NP_000094, NP_112503
Pathways Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis
Application Notes

DS WB Results: Approx 50-55 kDa band observed in Human Placenta lysates (calculated MW of 57.9 kDa according to NP_000094.2 and NP_112503.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

DS IHC Results: Paraffin embedded Human Placenta. Recommended concentration: 4 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (Internal Region) antibody (ABIN238672) ABIN238672 (4µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen ret...
Image no. 2 for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (Internal Region) antibody (ABIN238672) ABIN238672 (0.1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer)...