Gelsolin antibody (AA 763-775)

Catalog No. ABIN3031036

This Rabbit Polyclonal antibody specifically detects Gelsolin in WB, ICC and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Gelsolin antibody (AA 763-775) (ABIN3031036)

Target: Gelsolin (GSN)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Gelsolin Antibody

Binding Specificity: AA 763-775

Purification: Antigen affinity

Immunogen: Amino acids 763-775 (WDDDYWSVDPLDR-human) were used as the immunogen for this Gelsolin antibody (100% rat homology).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Gelsolin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Gelsolin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: Gelsolin

Background: Gelsolin also known as GNS is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. It is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. Gelsolin is also known as brevin, or actin-depolymerizing factor, it is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. It may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of Gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.

UniProt: P06396

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy