GLI3 antibody (N-Term)

Catalog No. ABIN3031041

This anti-GLI3 antibody is a Rabbit Polyclonal antibody detecting GLI3 in WB. Suitable for Human, Mouse and Rat.

Quick Overview for GLI3 antibody (N-Term) (ABIN3031041)

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GLI3 Antibody

Binding Specificity: N-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human Gli-kruppel family member 3 (SNEDESPGQTYHRERRNA) was used as the immunogen for this Gli3 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Gli3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. There are two isoforms of Gli-kruppel family member 3: the 170-190KD full length form and a truncated ~80KD form. This antibody will detect both, depending on the testing material.\. Western blot: 0.5-1 μg/mL (1)

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Gli3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: Gli3

Background: Gli-kruppel family member 3 is a member of the zinc finger family related to Kruppel, a protein family that is known to regulate development in Drosophila. The gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000D) shows sequence-specific DNA binding. Gli3 is homologous to the Drosophila cubitus interruptus (ci) gene product(Ci), which regulates the Patched (pct), Gooseberry (gsb), and Decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate Gli3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of Gli3 repressor suggested specialization of the protein and its products for negative hedgehog pathway regulation.

UniProt: P10071

Pathways: Hedgehog Signaling