HSPH1 antibody (AA 713-733)

Catalog No. ABIN3031140

This anti-HSPH1 antibody is a Rabbit Polyclonal antibody detecting HSPH1 in WB, IHC (p) and IHC (fro). Suitable for Human, Rat and Mouse.

Quick Overview for HSPH1 antibody (AA 713-733) (ABIN3031140)

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HSPH1 Antibody

Binding Specificity: AA 713-733

Purification: Antigen affinity

Immunogen: Amino acids 713-733 (EVMEWMNNVMNAQAKKSLDQD-human) were used as the immunogen for this HSP105 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the HSP105 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Hsp105 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for HSPH1

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Alternative Name: HSP105

Background: Heat shock protein 105, also called HSPH1 or HSP110, is a protein that in humans is encoded by the HSPH1 gene. Immunohistochemical analysis localizes HSP105 mainly in the cytoplasm. Database analysis indicates that both HSP105 isoforms are highly conserved during evolution. By analysis of radiation hybrids and human/rodent hybrid cell lines, the HSPH1 gene is mapped to chromosome 13. Both HSP105alpha and HSP105beta are upregulated in HeLa cells exposed to heat shock. HSP105alpha, but not HSP105beta, is also upregulate in response to other cell stresses. Following heat shock, HSP105 relocalizes from a cytoplasmic to perinuclear position. Additionally, it may constitute a major determinant for both prognosis and treatment response in colorectal cancer.

UniProt: Q92598