LCAT antibody (AA 300-313)
Quick Overview for LCAT antibody (AA 300-313) (ABIN3031596)
Target
See all LCAT AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 300-313
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Purification
- Antigen affinity
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Immunogen
- Amino acids 300-313 (RDFQRFFADLHFEE-human) were used as the immunogen for this LCAT antibody.
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Isotype
- IgG
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Application Notes
- The stated application concentrations are suggested starting amounts. Titration of the LCAT antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL
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Restrictions
- For Research Use only
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Buffer
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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Storage
- -20 °C
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Storage Comment
- After reconstitution, the LCAT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
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Alternative Name
- LCAT
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Background
- LCAT (Lecithin: Cholesterol Acyltransferase), is an enzyme that converts free cholesterol into cholesteryl ester. Azoulay et al.(1987) used a cDNA clone corresponding to LCAT to assign the locus to 16q22 through the analysis of DNA from somatic cell hybrids and in situ hybridization. LCAT plays an important role in lipoprotein metabolism, especially in the process termed reverse cholesterol transport The enzyme is synthesized in the liver and circulates in blood plasma as a complex with components of high density lipoprotein(HDL). Cholesterol from peripheral cells is transferred to HDL particles, esterified through the action of LCAT on HDL, and incorporated into the core of the lipoprotein. The cholesterol ester is thereby transported to the liver(Jonas, 2000).
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UniProt
- P04180
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Pathways
- Lipid Metabolism
Target
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