NME2 antibody (AA 137-152)

Catalog No. ABIN3032112

This anti-NME2 antibody is a Rabbit Polyclonal antibody detecting NME2 in WB, IHC (p) and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for NME2 antibody (AA 137-152) (ABIN3032112)

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NME2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-NME2 Antibody

Binding Specificity: AA 137-152

Purification: Antigen affinity

Immunogen: Amino acids 137-152 (EELVDYKSCAHDWVYE-human) were used as the immunogen for this NME2 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the NME2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the NME2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for NME2

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Alternative Name: NME2

Background: NME2 (NME/NM23 nucleoside diphosphate kinase 2) also called non-metastatic cells 2, protein (NM23B) expressed in, NM23-H2, NM23B or NDPKB, is identical to the beta subunit of human erythrocyte NDP kinase. Using Northern blot analysis, Masse et al.(2002) detected high expression of mouse NME2, which they called nm23-M2, in heart, liver, and kidney, with intermediate expression in skeletal muscle. In situ hybridization of 15-day postcoitum mouse embryos showed ubiquitous expression. The mouse and human genes contain 5 exons and span about 6.0 kb. The NME2 gene is mapped on 17q21.33. Srivastava et al.(2006) concluded that histidine phosphorylation regulates KCa3.1 channel activity and that NME2 is critical to the channel activity and the activation of CD4 T cells.

UniProt: P22392