PEBP1 antibody (N-Term)

Catalog No. ABIN3032217

This anti-PEBP1 antibody is a Rabbit Polyclonal antibody detecting PEBP1 in WB and IHC (p). Suitable for Mouse and Rat.

Quick Overview for PEBP1 antibody (N-Term) (ABIN3032217)

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PEBP1 Antibody

Binding Specificity: N-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of mouse PBP (QEVDEPPQHALRVDY) was used as the immunogen for this PBP antibody (100% rat homology).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the PBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the PBP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Alternative Name: PBP

Background: Phosphatidylethanolamine-binding protein inhibits the phosphorylation and activation of MEK by RAF1. PBP coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. Overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PBP expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal expression correlated perfectly with the onset of apoptosis by Chatterjee et al(2004). Depletion of the protein decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.

UniProt: P70296

Pathways: Feeding Behaviour