Prolactin Receptor antibody (C-Term)

Catalog No. ABIN3032320

This Rabbit Polyclonal antibody specifically detects Prolactin Receptor in WB and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Prolactin Receptor antibody (C-Term) (ABIN3032320)

Target: Prolactin Receptor (PRLR)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prolactin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Prolactin Receptor Antibody

Binding Specificity: C-Term

Purification: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of mouse Prolactin Receptor (QLGRLDYLDPTCFMH) was used as the immunogen for this Prolactin Receptor antibody (100% rat homology).

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the Prolactin Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the Prolactin Receptor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for Prolactin Receptor

Target: Prolactin Receptor (PRLR)

Alternative Name: Prolactin Receptor

Background: PRLR (Prolactin Receptor) is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al.(1989, 1990) demonstrated that the gene resides in the same chromosomal region as the growth hormone receptor (GHR) gene, which has been mapped to 5p13-p12. Cunningham et al.(1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to GHR or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues(histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR (conserved in all PRLRs but not GHRs) are likely zinc-ion ligands.

UniProt: Q08501

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus