PKC iota antibody (AA 21-214)

Catalog No. ABIN3032379

The Rabbit Polyclonal anti-PKC iota antibody (ABIN3032379) specifically detects PKC iota in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PKC iota antibody (AA 21-214) (ABIN3032379)

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC iota antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKC iota Antibody

Binding Specificity: AA 21-214

Purification: Antigen affinity

Immunogen: Human partial recombinant protein (AA 21-214) was used as the immunogen for this PKC iota antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting amounts. Titration of the PKC iota antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the PKC iota antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for PKC iota

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Alternative Name: PKC iota

Background: Protein kinase C iota, also called PKCI, is an enzyme that in humans is encoded by the PRKCI gene. This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where it phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. PRKCI is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. It is also a hedgehog target gene that forms a positive feedback loop with GLI and exists at increased levels in basal cell carcinomas.

Gene ID: 5584

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation