SCO2 antibody (C-Term)

Catalog No. ABIN303242

The Rabbit Polyclonal anti-SCO2 antibody is suitable to detect SCO2 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for SCO2 antibody (C-Term) (ABIN303242)

Target: SCO2 (SCO2 Cytochrome C Oxidase Assembly Protein (SCO2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SCO2 Antibody

Binding Specificity: C-Term

Specificity: Recognizes SCO Cytochrome Oxidase Deficient Homolog 2 (SCO2).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: SCO2 antibody was raised against Synthetic Peptide (-KLH conjugated) corresponding to 19 Amino Acid peptide from near the carboxy terminus of Human SCO2.

Application Details

Application Notes: Western Blot. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). This SCO2 antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate Buffered Saline PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Target Details for SCO2

Target: SCO2 (SCO2 Cytochrome C Oxidase Assembly Protein (SCO2))

Alternative Name: SCO2

Background: Mammalian cytochrome c oxidase (COX) catalyzes the transfer of reducing equivalents from Cytochrome C to molecular oxygen and pumps protons across the inner mitochondrial membrane. In yeast, 2 related COX assembly genes, SCO1 and SCO2 (synthesis of Cytochrome C Oxidase), enable subunits 1 and 2 to be incorporated into the holoprotein. This gene is the human homolog of the yeast SCO2 gene.Synonyms: Protein SCO2 homolog mitochondrial

Gene ID: 9997

NCBI Accession: NP_001162580

UniProt: O43819

Pathways: Transition Metal Ion Homeostasis, Warburg Effect