XAF1 antibody (C-Term)

Catalog No. ABIN303317

The Rabbit Polyclonal anti-XAF1 antibody is suitable to detect XAF1 in samples from Human and Mouse. It has been validated for WB and IHC (p).

Quick Overview for XAF1 antibody (C-Term) (ABIN303317)

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-XAF1 Antibody

Binding Specificity: C-Term

Specificity: This antibody recognises XIAP-Associated Factor 1.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic Peptide corresponding to amino acids at the C-terminus of human XAF-1.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunohistochemistry (2 μg/mL). Western Blot (0.5-2 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate Buffered Saline PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C to -70 °C for longer. Dilute only prior to immediate use.

Target Details for XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Alternative Name: XAF1

Background: XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-a2 and IFN-b can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.Synonyms: BIRC4-binding protein, BIRC4BP, XIAP-associated factor 1, XIAPAF1

Gene ID: 54739

NCBI Accession: NP_059993

UniProt: Q6GPH4