CD235a/GYPA antibody (N-Term) (FITC)

Catalog No. ABIN303572

The Mouse Monoclonal anti-CD235a/GYPA antibody has been validated for FACS. It is suitable to detect CD235a/GYPA in samples from Human.

Quick Overview for CD235a/GYPA antibody (N-Term) (FITC) (ABIN303572)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: HIR2

Product Details anti-CD235a/GYPA Antibody (FITC)

Binding Specificity: N-Term

Specificity: This antibody recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.

Immunogen: Synthetic peptide (Human, N-terminal)

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10^6 cells in a suspension.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline (PBS) containing 15 mM Sodium Azide and 0.2 % (w/v) high-grade protease free BSA as a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: CD235a / GYPA

Background: CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.Synonyms: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha

Gene ID: 2993

UniProt: P02724

Pathways: Maintenance of Protein Location