The Sheep Polyclonal anti-Emerin antibody has been validated for WB, IF, ICC and EM. It is suitable to detect Emerin in samples from Mouse, Rat and Hamster.
Affinity purified on GST-sepharose column with attached antigen
Immunogen
Synthetic peptide: MSKSFRRPGT SLVDADDTFH HQVRDDIFSS SEEEGKDRER PIYGRDSAYQ SIAEYRPISN VSRSSLGLSY, corresponding to internal sequence amino acids 114-183 of Rat Emerin, with N terminal proprietary tag. (P50402)
Optimal antibody dilution should be determined by titration, however as a guideline try WB: 1/1000. Predicted molecular weight: 30 kDa.ICC/IF: 1/100.Electron MicroscopyEM: 1/50. (Rat)