MICB antibody (N-Term)

Catalog No. ABIN3042366

Product Details anti-MICB Antibody

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Binding Specificity: AA 23-48, N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MICB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purpose: Anti-MICB Antibody Picoband®

Sequence: AEPHSLRYNL MVLSQDESVQ SGFLAE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MICB Antibody Picoband® (ABIN3042366). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MICB.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bahram S, Bresnahan M, Geraghty DE, Spies T (Aug 1994). "A second lineage of mammalian major histocompatibility complex class I genes". Proc Natl Acad Sci U S A 91 (14): 6259-63. 2. "Entrez Gene: MICB MHC class I polypeptide-related sequence B". 3. Leelayuwat C, Townend DC, Degli-Esposti MA et al. (1994). "A new polymorphic and multicopy MHC gene family related to nonmammalian class I.".Immunogenetics 40 (5): 339-51.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MICB

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Alternative Name: MICB (MICB Products)

Background:

Synonyms: MHC class I polypeptide-related sequence B,MIC-B,MICB ,PERB11.2 ,

Tissue Specificity: Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue. .

Background: MHC class I polypeptide-related sequence B is a protein that in humans is encoded by the MICB gene. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules, however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants.

Sequence Similarities: Belongs to the MHC class I family. MIC subfamily.

Molecular Weight: 60-65 kDa

Gene ID: 4277

UniProt: Q29980

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response