Myeloperoxidase antibody (AA 406-745)

Catalog No. ABIN3042368

The Rabbit Polyclonal anti-Myeloperoxidase antibody has been validated for WB, IHC and FACS. It is suitable to detect Myeloperoxidase in samples from Human. There are 4+ publications available.

Quick Overview for Myeloperoxidase antibody (AA 406-745) (ABIN3042368)

Target: Myeloperoxidase (MPO)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myeloperoxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Myeloperoxidase Antibody

Binding Specificity: AA 406-745

Purpose: Anti-Myeloperoxidase/MPO Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Myeloperoxidase/MPO Antibody Picoband® (ABIN3042368). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Myeloperoxidase recombinant protein (Position:S406-S745). Human Myeloperoxidase shares 90% amino acid (aa) sequence identity with mouse Myeloperoxidase.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Klebanoff, S. J. : Myeloperoxidase. Proc. Assoc. Am. Phys. 111: 383-389, 1999. 2. Murao, S.-I., Stevens, F. J., Ito, A., Huberman, E. : Myeloperoxidase: a myeloid cell nuclear antigen with DNA-binding properties. Proc. Nat. Acad. Sci. 85: 1232-1236, 1988. 3. Nauseef, W. M., Olsson, I., Arnljots, K. : Biosynthesis and processing of myeloperoxidase--a marker for myeloid cell differentiation. Europ. J. Haemat. 40: 97-110, 1988.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Myeloperoxidase antibody (ABIN3042368)

Wei, Kong, Li, Guan, Wang, Miao: "Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway." in: Scientific reports, Vol. 7, Issue 1, pp. 717, (2018) (PubMed).

Xu, Zhang, He, Li, Gao, Liu: "Lactobacillus acidophilus alleviates pouchitis after ileal pouch-anal anastomosis in rats." in: World journal of gastroenterology, Vol. 23, Issue 26, pp. 4735-4743, (2018) (PubMed).

Wang, Liu, Liu, Wang, Fan, Ma, Jin, Xiang, Huang, Zhang, Xu, Yin, He: "Interleukin-17A Aggravates Middle Ear Injury Induced by Streptococcus pneumoniae through the p38 Mitogen-Activated Protein Kinase Signaling Pathway." in: Infection and immunity, Vol. 85, Issue 10, (2017) (PubMed).

Yu, Chen, Wang, Kuang, Liu, Zhang, Du: "Neuroprotective effect of kaempferol glycosides against brain injury and neuroinflammation by inhibiting the activation of NF-?B and STAT3 in transient focal stroke." in: PLoS ONE, Vol. 8, Issue 2, pp. e55839, (2013) (PubMed).

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: MPO

Background:

Synonyms: Myeloperoxidase,MPO,1.11.2.2,Myeloperoxidase,89 kDa myeloperoxidase,84 kDa myeloperoxidase,Myeloperoxidase light chain,Myeloperoxidase heavy chain,MPO,

Tissue Specificity: Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. .

Background: Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function. The standard product used in this kit is the product of gene recombination, consisting of 697 (A49-S745) amino acids with the molecular mass of 80KDa.

Sequence Similarities: Belongs to the peroxidase family. XPO subfamily.

Molecular Weight: 60-65 kDa, 80-90 kDa

Gene ID: 4353

UniProt: P05164

Pathways: Chromatin Binding