FMO2 antibody (N-Term)

Catalog No. ABIN3042397

Product Details anti-FMO2 Antibody

Target: FMO2 (Flavin Containing Monooxygenase 2 (Non-Functional) (FMO2))

Binding Specificity: AA 78-115, N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FMO2 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-FMO2 Antibody Picoband®

Sequence: FPNFLHNSKL LEYFRIFAKK FDLLKYIQFQ TTVLSVRK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FMO2 Antibody Picoband® (ABIN3042397). Tested in WB applications. This antibody reacts with Human;Mouse;Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human FMO2, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: FMO2 flavin containing monooxygenase 2 (non-functional)". 2. Dolphin CT, Shephard EA, Povey S, Smith RL, Phillips IR (Nov 1992). "Cloning, primary sequence and chromosomal localization of human FMO2, a new member of the flavin-containing mono-oxygenase family".Biochem J. 287. ( Pt 1): 261-7. 3. Dolphin CT, Beckett DJ, Janmohamed A, Cullingford TE, Smith RL, Shephard EA, Phillips IR (Dec 1998). "The flavin-containing monooxygenase 2 gene (FMO2) of humans, but not of other primates, encodes a truncated, nonfunctional protein". J Biol Chem 273 (46): 30599-607.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FMO2

Target: FMO2 (Flavin Containing Monooxygenase 2 (Non-Functional) (FMO2))

Alternative Name: FMO2 (FMO2 Products)

Background:

Synonyms: Dimethylaniline monooxygenase [N-oxide-forming] 2,1.14.13.8,Dimethylaniline oxidase 2,FMO 1B1,Pulmonary flavin-containing monooxygenase 2,FMO 2,FMO2,

Tissue Specificity: Expressed in lung (at protein level). Expressed predominantly in lung, and at a much lesser extent in kidney. Also expressed in fetal lung, but not in liver, kidney and brain. .

Background: Dimethylaniline monooxygenase [N-oxide-forming] 2 is an enzyme that in humans is encoded by the FMO2 gene. This gene encodes a flavin-containing monooxygenase family member. It is an NADPH-dependent enzyme that catalyzes the N-oxidation of some primary alkylamines through an N-hydroxylamine intermediate. However, some human populations contain an allele (FMO2*2A) with a premature stop codon, resulting in a protein that is C-terminally-truncated, has no catalytic activity, and is likely degraded rapidly. This gene is found in a cluster with other related family members on chromosome 1. Alternative splicing results in multiple transcript variants.

Molecular Weight: 61 kDa

Gene ID: 2327

UniProt: Q99518