FURIN antibody (AA 591-794)

Catalog No. ABIN3042409

This Rabbit Polyclonal antibody specifically detects FURIN in WB. It exhibits reactivity toward Human.

Quick Overview for FURIN antibody (AA 591-794) (ABIN3042409)

Target: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FURIN antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FURIN Antibody

Binding Specificity: AA 591-794

Purpose: Anti-Furin Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Furin Antibody Picoband® (ABIN3042409). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Furin recombinant protein (Position: T591-L794). Human Furin shares 88% amino acid (aa) sequence identity with both mouse and rat Furin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Wise RJ, Barr PJ, Wong PA, Kiefer MC, Brake AJ, Kaufman RJ (Jan 1991). "Expression of a human proprotein processing enzyme: correct cleavage of the von Willebrand factor precursor at a paired basic amino acid site". Proc Natl Acad Sci U S A 87(23): 9378-82. 2. Hallenberger S, Bosch V, Angliker H, Shaw E, Klenk HD, Garten W (November 1992). "Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gp160". Nature 360 (6402): 358-61. 3. Thomas G (October 2002). "Furin at the cutting edge: from protein traffic to embryogenesis and disease". Nat. Rev. Mol. Cell Biol. 3 (10): 753-66.doi:10.1038/nrm934. 4. Roebroek, A. J. M., Schalken, J. A., Leunissen, J. A. M., Onnekink, C., Bloemers, H. P. J., Van de Ven, W. J. M. Evolutionary conserved close linkage of the c-fes/fps proto-oncogene and genetic sequences encoding a receptor-like protein. EMBO J. 5: 2197-2202, 1986.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for FURIN

Target: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Alternative Name: FURIN

Background:

Synonyms: Furin,3.4.21.75,Dibasic-processing enzyme,Paired basic amino acid residue-cleaving enzyme,PACE,FURIN,FUR, PACE, PCSK3,

Tissue Specificity: Seems to be expressed ubiquitously.

Background: Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The Furin gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance.

Sequence Similarities: Belongs to the peptidase S8 family. Furin subfamily.

Molecular Weight: 87 kDa

Gene ID: 5045

UniProt: P09958

Pathways: Notch Signaling, Neurotrophin Signaling Pathway