Glucose-6-Phosphate Dehydrogenase antibody (AA 315-515)

Catalog No. ABIN3042413

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Binding Specificity: AA 315-515

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband® (ABIN3042413). Tested in WB applications. This antibody reacts with Human;Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human G6PD recombinant protein (Position: E315-L515). Human G6PD shares 95% and 96% amino acid (aa) sequences identity with mouse and rat G6PD, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. Aster J, Kumar V, Robbins SL, Abbas AK, Fausto N, Cotran RS (2010). Robbins and Cotran pathologic basis of disease. Saunders/Elsevier. pp. Kindle Locations 33340-33341. 2. Takizawa, T., Huang, I.-Y., Ikuta, T., Yoshida, A. Human glucose-6-phosphate dehydrogenase: primary structure and cDNA cloning. Proc. Nat. Acad. Sci. 83: 4157-4161, 1986.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Glucose-6-Phosphate Dehydrogenase antibody (ABIN3042413)

Hu, Zhang, Tang, Su, Li, Chen, Zhang, Cai, Zhu: "Variant G6PD levels promote tumor cell proliferation or apoptosis via the STAT3/5 pathway in the human melanoma xenograft mouse model." in: BMC cancer, Vol. 13, Issue 1, pp. 251, (2014) (PubMed).

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: G6PD (G6PD Products)

Background:

Synonyms: Glucose-6-phosphate 1-dehydrogenase,G6PD,1.1.1.49,G6PD,

Tissue Specificity: Isoform Long is found in lymphoblasts, granulocytes and sperm.

Background: Glucose-6-phosphate dehydrogenase, also known as G6PD or G6PDH, is an enzyme that in humans is encoded by the G6PD gene. It is mapped to Xq28. G6PD plays a key role in the production of ribose 5-phosphate and the generation of NADPH in the hexose monophosphate pathway. Because this pathway is the only NADPH-generation process in mature red cells, which lack the citric adid cycle, a genetic deficiency of G6PD is often associated with adverse physiologic effects. It has been found that aldosterone decreased G6PD expression and activity, resulting in increased oxidant stress and decreased nitric oxide levels, similar to what is observed in G6PD-deficient endothelial cells.

Sequence Similarities: Belongs to the glucose-6-phosphate dehydrogenase family.

Molecular Weight: 59 kDa

Gene ID: 2539

UniProt: P11413

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones