GATA5 antibody (AA 1-397)

Catalog No. ABIN3042418

This Rabbit Polyclonal antibody specifically detects GATA5 in WB. It exhibits reactivity toward Human.

Quick Overview for GATA5 antibody (AA 1-397) (ABIN3042418)

Target: GATA5 (GATA Binding Protein 5 (GATA5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GATA5 Antibody

Binding Specificity: AA 1-397

Purpose: Anti-GATA5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GATA5 Antibody Picoband® (ABIN3042418). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GATA5 recombinant protein (Position: M1-A397). Human GATA5 shares 75% amino acid (aa) sequence identity with mouse GATA5.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Gao, X., Sedgwick, T., Shi, Y.-B., Evans, T. Distinct functions are implicated for the GATA-4, -5, and -6 transcription factors in the regulation of intestine epithelial cell differentiation. Molec. Cell. Biol. 18: 2901-2911, 1998. 2. "Entrez Gene: GATA5 GATA binding protein 5"

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GATA5

Target: GATA5 (GATA Binding Protein 5 (GATA5))

Alternative Name: GATA5

Background:

Synonyms: Transcription factor GATA-5,GATA-binding factor 5,GATA5 ,

Tissue Specificity: T-cells and endothelial cells.

Background: Transcription factor GATA-5, also named GATAS or GATA5, is a protein that in humans is encoded by the GATA5 gene. It is mapped to 20q13.33. The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. In addition, the expression of GATA5 increased in a human colon cancer cell line within 2 hours of exposure to sodium butyrate, which induces differentiation.

Sequence Similarities: Contains 2 GATA-type zinc fingers.

Molecular Weight: 50 kDa

Gene ID: 140628