Glutamate Receptor 3 antibody (AA 29-360)

Catalog No. ABIN3042428

The Rabbit Polyclonal anti-Glutamate Receptor 3 antibody has been validated for WB. It is suitable to detect Glutamate Receptor 3 in samples from Human, Rat and Mouse.

Quick Overview for Glutamate Receptor 3 antibody (AA 29-360) (ABIN3042428)

Target: Glutamate Receptor 3 (GRIA3) (Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutamate Receptor 3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Glutamate Receptor 3 Antibody

Binding Specificity: AA 29-360

Purpose: Anti-Glutamate receptor 3/GRIA3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Glutamate receptor 3/GRIA3 Antibody Picoband® (ABIN3042428). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GRIA3 recombinant protein (Position: G29-M360). Human GRIA3 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. "Entrez Gene: GRIA3 glutamate receptor, ionotrophic, AMPA 3 2. Hirbec, Hélène, Perestenko Olga, Nishimune Atsushi, Meyer Guido, Nakanishi Shigetada, Henley Jeremy M, Dev Kumlesh K (May 2002). "The PDZ proteins PICK1, GRIP, and syntenin bind multiple glutamate receptor subtypes. Analysis of PDZ binding motifs". J. Biol. Chem. (United States) 277 (18): 15221-4. 3. McNamara JO, Eubanks JH, McPherson JD, Wasmuth JJ, Evans GA, Heinemann SF (Jul 1992). "Chromosomal localization of human glutamate receptor genes". J Neurosci 12 (7): 2555-62.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Glutamate Receptor 3

Target: Glutamate Receptor 3 (GRIA3) (Glutamate Receptor, Ionotropic, AMPA 3 (GRIA3))

Alternative Name: GRIA3

Background:

Synonyms: Glutamate receptor 3,GluR-3,AMPA-selective glutamate receptor 3,GluR-C,GluR-K3,Glutamate receptor ionotropic, AMPA 3,GluA3,GRIA3,GLUR3, GLURC,

Tissue Specificity: Widely expressed in brain.

Background: Glutamate receptor 3 is a protein that in humans is encoded by the GRIA3 gene. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. It is mapped to Xq25. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists.

Sequence Similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA3 subfamily.

Molecular Weight: 101 kDa

Gene ID: 2892

UniProt: P42263

Pathways: PI3K-Akt Signaling, cAMP Metabolic Process, Synaptic Membrane