HHEX antibody (Middle Region)

Catalog No. ABIN3042444

This anti-HHEX antibody is a Rabbit Polyclonal antibody detecting HHEX in WB. Suitable for Human, Mouse and Rat.

Quick Overview for HHEX antibody (Middle Region) (ABIN3042444)

Target: HHEX (Hematopoietically Expressed Homeobox (HHEX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HHEX antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HHEX Antibody

Binding Specificity: AA 146-180, Middle Region

Purpose: Anti-Hex/HHEX Antibody Picoband®

Sequence: NDQTIELEKK FETQKYLSPP ERKRLAKMLQ LSERQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Hex/HHEX Antibody Picoband® (ABIN3042444). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Hex, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. D'Elia, A. V., Tell, G., Russo, D., Arturi, F., Puglisi, F., Manfioletti, G., Gattei, V., Mack, D. L., Cataldi, P., Filetti, S., Di Loreto, C., Damante, G. Expression and localization of the homeodomain-containing protein HEX in human thyroid tumors. J. Clin. Endocr. Metab. 87: 1376-1383, 2002. 2. Hromas, R., Radich, J., Collins, S. PCR cloning of an orphan homeobox gene (PRH) preferentially expressed in myeloid and liver cells. Biochem. Biophys. Res. Commun. 195: 976-983, 1993.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HHEX

Target: HHEX (Hematopoietically Expressed Homeobox (HHEX))

Alternative Name: HHEX

Background:

Synonyms: Hematopoietically-expressed homeobox protein HHEX,Homeobox protein HEX,Homeobox protein PRH,HHEX,HEX, PRH, PRHX,

Tissue Specificity: Liver and promyelocytic leukemia cell line HL- 60. .

Background: Hematopoietically-expressed homeobox protein HHEX is a protein that in humans is encoded by the HHEX gene. Homeobox genes are members of a family of transcription factors that regulate tissue development in many different organisms. Hromas et al. (1993) set out to identify homeobox genes that might play a role in hematopoiesis. And using somatic cell hybrid analysis, they mapped the HHEX gene to chromosome 10, where the HOX11 gene is located. Homeobox genes are involved in neoplastic transformation of both epithelial and hemopoietic tissues. The divergent homeobox gene HEX is expressed in the anterior visceral endoderm during early mouse development and in some adult tissues of endodermal origin, including liver and thyroid. D'Elia et al.'s findings suggested that regulation of HEX entry in the nucleus of thyrocytes may represent a critical step during human thyroid tumorigenesis.

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Molecular Weight: 40 kDa

Gene ID: 3087

UniProt: Q03014